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Probst, I. ; Schlegel, H.G.

Studies on a gram-positive hydrogen bacterium, Nocardia opaca strain 1b - II. Enzyme formation and regulation under the influence of hydrogen or fructose as growth substrates.

Arch. Microbiol. 88, 319-330 (1973)
Verlagsversion DOI
In Nocardia opaca strain 1b the key enzymes of the Entner-Doudoroff pathway, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase, are inducible, while glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase are constitutively formed. In autotrophically grown cells the fructose diphosphate aldolase has six-fold specific activity compared to fructose- or gluconate-grown cells. Molecular hydrogen represses the adaptation to fructose, glucose, or gluconate and inhibits the utilization of glucose or fructose. Glucose-6-phosphate dehydrogenase has been purified 22fold. It utilizes either NADP of NAD as coenzyme; it requires magnesium ions for maximal activity. The dehydrogenation reaction is competitively inhibited by rather high concentrations (2-6 mM) of ATP, and in a similar fashion by phosphoenolpyruvate. Homotropic or heterotropic interactions between substrate molecules were not detected. The effect of ATP is relieved by equimolar concentrations of magnesium.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Zeitschrift Archives of Microbiology
Quellenangaben Band: 88, Heft: 4, Seiten: 319-330 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Mikrobiologie