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Guertler, A.* ; Kraemer, A. ; Roessler, U.* ; Hornhardt, S.* ; Kulka, U.* ; Mörtl, S. ; Friedl, A.A.* ; Illig, T. ; Wichmann, H.-E. ; Gomolka, M.

The WST survival assay: An easy and reliable method to screen radiation-sensitive individuals.

Radiat. Prot. Dosim. 143, 487-490 (2011)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
An easy, fast and reliable method was developed to screen hundreds of Epstein-Barr virus-transformed cell lines (lymphoblastoid cell lines, LCLs) for radiation sensitivity that were generated from lymphocytes isolated from young lung cancer patients. The WST-1 test explores the metabolic activity of the mitochondria as an indicator for the vital status of cells. Cell proliferation as well as indirect cell death can be quantified by this method on a large scale in microtiter plates. Cell survival was measured at 24- and 48-h post-irradiation with 10 Gy ((137)Cs source) by the WST-1 assay and Trypan blue staining. To set up the experimental screening conditions and to establish a positive and a negative control, an ATM-mutated cell line from a radiation-sensitive ATM patient and an ATM proficient cell line from a healthy brother were compared. An optimal differentiation between the two cell lines was demonstrated for 10 Gy and 24- and 48-h cell growth after irradiation. Upon screening 120 LCLs of young lung cancer patients under these conditions, 5 of them were found to be radiation sensitive to a high degree of statistical significance. The results have been confirmed by a second laboratory by means of Trypan blue testing. The WST-1 test represents an efficient and reliable method by means of screening for radiation-sensitive cell lines.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Lung-Cancer; Radiosensitivity
ISSN (print) / ISBN 0144-8420
e-ISSN 1742-3406
Zeitschrift Radiation Protection Dosimetry
Quellenangaben Band: 143, Heft: 2-4, Seiten: 487-490 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Oxford
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
Institute of Epidemiology (EPI)
Research Unit Molecular Epidemiology (AME)