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Töwe, S.* ; Kleineidam, K. ; Schloter, M.

Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples.

J. Microbiol. Methods 82, 338-341 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
High and comparable efficiency values are the key for reliable quantification of target genes from environmental samples using real-time PCR. Therefore it was the aim of this study to investigate if PCR amplification efficiencies of plasmid DNA used for the calculation of standard curves (i) remain constant along a logarithmic scale of dilutions and (ii) if these values are comparable to those of DNA extracted from environmental samples. It could be shown that comparable efficiency values within the standards cannot be achieved using log scale serial dilutions and a comparison of gene copy numbers from DNA extracted from environmental samples and standard DNA extracted from plasmids is only possible in a very small interval.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Quantitative real-time PCR; Amplification efficiency; Standard curve; LinRegPCR; Nitrogen cycle; Functional genes
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Zeitschrift Journal of Microbiological Methods
Quellenangaben Band: 82, Heft: 3, Seiten: 338-341 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)