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Graw, J. ; Liebstein, A. ; Pietrowski, D. ; Schmitt-John, T. ; Werner, T.

Genomic sequences of murine γB- and γC-crystallm-encoding genes: promoter analysis and complete evolutionary pattern of mouse, rat and human γ-crystallins.

Gene 136, 145-156 (1993)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The murine genes, γB-cry and γC-cry, encoding the γB- and γC-crystallins, were isolated from a genomic DNA library. The complete nucleotide (nt) sequences of both genes were determined from 661 and 711 bp, respectively, upstream from the first exon to the corresponding polyadenylation sites, comprising more than 2650 and 2890 bp, respectively. The new sequences were compared to the partial cDNA sequences available for the murine γB-cry and γC-cry, as well as to the corresponding genomic sequences from rat and man, at both the nt and predicted amino acid (aa) sequence levels. In the γB-cry promoter region, a canonical CCAAT-box, a TATA-box, putative NF-I and C/EBP sites were detected. An R-repeat is inserted 366 bp upstream from the transcription start point. In contrast, the γC-cry promoter does not contain a CCAAT-box, but some other putative binding sites for transcription factors (AP-2, UBP-1, LBP-1) were located by computer analysis. The promoter regions of all six γ-cry from mouse, rat and human, except human ψγF-cry, were analyzed for common sequence elements. A complex sequence element of about 70-80 bp was found in the proximal promoter, which contains a γ-cry-specific and almost invariant sequence (crygpel) of 14 nt, and ends with the also invariant TATA-box. Within the complex sequence element, a minimum of three further features specific for the γA-, γB- and γD/E/F-cry genes can be defined, at least two of which were recently shown to be functional: In addition to these four sequence elements, a subtype-specific structure of inverted repeats with different-sized spacers can be deduced from the multiple sequence alignment. A phylogenetic analysis based on the promoter region, as well as the complete exon 3 of all γ-cry from mouse, rat and man, suggests separation of only five γ-cry subtypes (γA-, γB-, γC-, γD- and γE/F-cry) prior to species separation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Development ; Evolution ; Eye Lens ; Gene Expression ; Genomic Dna Library ; Sequence Analysis ; Silencer
ISSN (print) / ISBN 0378-1119
e-ISSN 1879-0038
Zeitschrift Gene
Quellenangaben Band: 136, Heft: 1-2, Seiten: 145-156 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)