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Schloter, M. ; Borlinghaus, R.T. ; Bode, W. ; Hartmann, A.

Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal scanning laser microscopy.

J. Microsc. 171, 173-177 (1993)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Azospirillum is a soil bacterium with plant growth-promoting potential. Strain-specific monoclonal antibodies (mAbs) which bind to different antigenic determinants of the bacterial cell surface with high specificity for A. brasilense Sp7 were used to monitor this bacterium in the rhizosphere of wheat plants. The mAbs were marked directly with the fluorochromes fluorescein (FITC) or tetramethylrhodamine (TRITC). With the labelled mAbs and focal scanning laser microscopy, an in situ identification and localization of this bacterium in root segments was possible.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Azospirillum ; Confocal Scanning Laser Microscopy ; Monoclonal Antibodies
ISSN (print) / ISBN 0022-2720
e-ISSN 1365-2818
Zeitschrift Journal of Microscopy
Quellenangaben Band: 171, Heft: 2, Seiten: 173-177 Artikelnummer: , Supplement: ,
Verlag Wiley
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)
Research Unit Microbe-Plant Interactions (AMP)