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Oxynos, K. ; Schladot, J.D.* ; Schmitzer, J.L. ; Backhaus, F.W.* ; Kettrup, A.

Characterisation of chlorinated hydrocarbons in a third generation candidate fish reference material (Limanda limanda) prepared under cryogenic conditions.

Fresenius Z. Anal. Chem. 345, 238-239 (1993)

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This paper describes the cryogenic preparation of 50 individual specimens of dab (Limanda limanda) from the Baltic Sea to produce a homogeneous fish material. 950 subsamples (8 g each) are stored in the gaseous phase over liquid nitrogen. Five series of analyses were randomly selected out of store. The results show that the obtained precision of 8 to 9% could be used as an indication unit for the deviation of the mean values of CHC concentrations from "true" values. If the analytical variability is 5%, the maximum inhomogeneity in the subsamples is 3-4%. It may be concluded that the subsamples of the candidate fish reference material (RM) are homogeneous enough for analytical purposes. Thus ESB (German Environmental Specimen Bank) material prepared in accordance with the standard operating procedure (SOP) [1] are valuable and appropriate reference materials. The prepared fish RM is suitable for the analysis of chlorinated hydrocarbons in fat containing marine samples.

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