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Large scale production and purification of human retrovirus-like particles related to the mouse mammary tumor virus.
FEMS Microbiol. Lett. 109, 289-296 (1993)
Human retrovirus-like particles related to mouse mammary tumor virus (MMTV) are secreted in a steroid-dependent manner by the breast cancer cell line T47D. We report the successful large scale production and purification of these particles from culture supernatants of T47D cells and describe the experimental conditions established for this purpose. Thus, mg amounts of particles were produced by large scale culturing of T47D cells in an autoharvesting roller bottle system and purified by differential centrifugation and continuous flow ultracentrifugation on density gradients with a 50% recovery and a 350-fold enrichment.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Human Mmtv-related Particles ; Large Scale Purification
ISSN (print) / ISBN
0378-1097
e-ISSN
1574-6968
Zeitschrift
FEMS Microbiology Letters
Quellenangaben
Band: 109,
Heft: 2-3,
Seiten: 289-296
Verlag
Wiley
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Virology (VIRO)