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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Immunoglobulin heavy-chain joining genes in the rat: Comparison with mouse and human.
Gene 102, 261-264 (1991)
We have cloned by the polymerase chain reaction a 2.1-kb fragment carrying heavy-chain joining (J(H)) gene segments and a part of the J(H)-C(μ) intron of the rat. Sequencing showed that the rat genome has four functional J(H) segments and that only a slight divergence has occurred after the separation of rat and mouse. A systematic sequence comparison between the two species and human revealed an additional J(H) pseudogene in rat and mouse 5' of J(H)1 which has not been described so far. The implications in evolutionary terms are discussed. In addition, we give an assessment of the misincorporation rate of the Taq polymerase.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
J Gene Segments ; Louvain Rat ; Nucleotide Sequence ; Polymerase Chain Reaction ; Pseudogene ; Recombinant Dna
ISSN (print) / ISBN
0378-1119
e-ISSN
1879-0038
Zeitschrift
Gene
Quellenangaben
Band: 102,
Heft: 2,
Seiten: 261-264
Verlag
Elsevier
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institut für Immunologie