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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Time-resolved fluorescence microscopy for measuring specific coenzymes in methanogenic bacteria.
Anal. Chim. Acta 163, 249-255 (1984)
Measurements of time-resolved photobleaching and nanosecond fluorescence decay from microscopic samples of methanogenic bacteria are reported. From cultures of Methanobacterium thermoautotrophicum and Methanosarcina barkeri, decay times of 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin, the fluorescence of F420 was bleached selectively, with a time constant of about 160 s, at an irradiation power density of 5 mW mm-2. Similar time constants were found for samples of sewage sludge containing methanogenic bacteria. Active and inactive bacterial cells could be differentiated by following the course of photobleaching.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0003-2670
e-ISSN
1873-4324
Zeitschrift
Analytica Chimica Acta
Quellenangaben
Band: 163,
Heft: C,
Seiten: 249-255
Verlag
Elsevier
Begutachtungsstatus
Peer reviewed
Institut(e)
Abteilung Biophysikalische Strahlenforschung