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Ziegler, I. ; Schwulera, U. ; Ellwart, J.W.

Pteridines are produced during interleukin 2-induced T-cell proliferation and modulate transmission of this signal.

Exp. Cell Res. 167, 531-538 (1986)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Pteridine levels of interleukin 2 (IL-2) receptor+ T-cell populations have been determined by HPLC after iodine oxidation; neopterin was monitored in the culture supernatants by radio-immunoassay. Upon addition of IL-2, cellular levels of biopterin and 6-hydroxymethylpterin rise transiently from 0.02 to 0.9 pmol/106 cells, cellular levels of neopterin from 1.5 to 4.1 pmol/106 cells. They peak at 8 and 13 h, respectively, after exposure to IL-2. Neopterin is not accumulated in the culture supernatant. DNA synthesis in T cells begins 10-12 h after adding the lymphokine and the portion of cells that undergo S-phase transition gradually increases during the subsequent 10 h. Entry into DNA synthesis phase is markedly accelerated if IL-2 is supplied together with tetrahydrobiopterin (0.8-1.6 x 10-6 M) and the kinetics of entry into the S-phase transition during the period of 6-20 h become linear. This indicates that tetrahydrobiopterin modulation of IL-2 activity (Ziegler, I. et al. Naturwiss 72 (1985) 330) is an early event occurring during IL-2 signal transmission.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 1090-2422
e-ISSN 0014-4827
Zeitschrift Experimental Cell Research
Quellenangaben Band: 167, Heft: 2, Seiten: 531-538 Artikelnummer: , Supplement: ,
Verlag Academic Press
Verlagsort Orlando, Fla.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Hämatologie