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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Direct measurement of pure absorbance spectra of living phototrophic microorganisms.
Biochim. Biophys. Acta-Gen. Subj. 538, 593-602 (1978)
The pure absorbance of turbid cell suspensions of various phototrophic microorganisms was determined by collecting the scattered light. A conventional spectrophotometer was used, equipped with an integrating sphere as receiver unit, which allowed precise measurements of the absorbance in the range from zero to 0.1. In the wavelength range 300-1100 nm, where photosynthesis occurs, light scattered only once by a bacterial cell retains predominantly the forward direction. This allows measurements of pure absorption, when the concentration of cells which the light has to pass through is small. For example, by comparison of measurements of pigmented and nonpigmented cell suspensions of Rhodopseudomonas acidophila, it was shown that the total sum of scattered light can be collected. The best results were obtained using cuvettes with a light path of 0.1 cm or 0.2 cm to measure cell suspensions of about 0.2 mg dry weight per ml. For R. acidophila this corresponds to 1-3 cell layers. Extinction-, absorbance- and scattering spectra for R. acidophila are presented, in addition to the absorbance spectra for Rhodospirillum rubrum, Aphanocapsa and Scenedesmus.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0304-4165
e-ISSN
1872-8006
Zeitschrift
Biochimica et Biophysica Acta - General Subjects
Quellenangaben
Band: 538,
Heft: 3,
Seiten: 593-602
Verlag
Elsevier
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institut für Mikrobiologie