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Buff, K. ; Bründl, A. ; Berndt, J.

Interaction of the insecticide 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane with the β-receptor adenylate cyclase system in Chang liver cell membranes.

Chem. Biol. Interact. 47, 337-346 (1983)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Interaction of the insecticide 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) with β-receptor binding and adenylate cyclase activity of biological membranes has been studied. Following exposure of cultured Chang liver cells to DDT, maximal binding of the catecholamine antagonist [125I]-iodohydroxybenzylpindol (HYP) to isolated cell membranes was decreased by 30% whereas the dissociation constant remained unchanged. Both basal activity and maximal isoproterenol-stimulated activity of adenylate cyclase were not altered. The isoproterenol concentration required for half-maximal stimulation of the enzyme was increased about 2-fold as was the agonist concentration required for half-maximal displacement of the antagonist HYP at the β-receptor binding site. Thus, coupling efficiency of hormone-stimulated adenylate cyclase activity was not influenced by the presence of DDT in these membranes. The data show that interaction of DDT with the β-receptor adenylate cyclase complex is restricted to the receptor component. Enzyme activity is directly linked to changes of agonist binding at the β-receptor. Interference of DDT with signal transuction via 'fluidization' of membrane lipids has not been detected.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0009-2797
e-ISSN 1872-7786
Zeitschrift Chemico-Biological Interactions
Quellenangaben Band: 47, Heft: 3, Seiten: 337-346 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed