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Kleineidam, K. ; Sharma, S. ; Kotzerke, A.* ; Heuer, H.* ; Thiele-Bruhn, S.* ; Smalla, K.* ; Wilke, B.-M.* ; Schloter, M.

Effect of sulfadiazine on abundance and diversity of denitrifying bacteria by determining nirK and nirS genes in two arable soils.

Microb. Ecol. 60, 703-707 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Sulfadiazine (SDZ) is an antibiotic frequently used in agricultural husbandry. Via manuring of excrements of medicated animals, the drug reaches the soil and might impair important biochemical transformation processes performed by microbes, e.g., the nitrogen turnover. We studied the effect of pig manure and SDZ-spiked pig manure on denitrifying bacteria by quantifying nirK and nirS nitrite reductase genes in two arable soils. Addition of manure entailed mainly an increase of nirK-harboring denitrifiers in both soils, whereas in the SDZ-amended treatments, primarily the nirS denitrifiers increased in abundance after the bioavailable SDZ had declined. However, the community composition of nirS nitrite reducers investigated by denaturing gradient gel electrophoresis did not change despite the observed alterations in abundance.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter PIG-MANURE; AGRICULTURAL SOILS; COMMUNITY; DENITRIFICATION; NITRIFICATION; INHIBITION; DYNAMICS; NITRATE; SAMPLES
ISSN (print) / ISBN 0095-3628
e-ISSN 1432-184X
Zeitschrift Microbial Ecology
Quellenangaben Band: 60, Heft: 4, Seiten: 703-707 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed