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Andrews, J.F. ; Martin-Bertram, H.* ; Hagen, U.

S1 nuclease-sensitive sites in yeast DNA: An assay for radiation-induced base damage.

Int. J. Radiat. Biol. 45, 497-504 (1984)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
DNA has been isolated from γirradiated yeast cells and then treated with the enzyme S1 nuclease. This enzyme cleaves DNA specifically at sites where localized denaturation has occurred and can therefore be used as a lesion probe to identify regions in the DNA where base-pairing has been disrupted. By analysing the number of single strand breaks, double strand breaks and alkali-labile sites in the DNA before and after treatment with S1 nuclease, it has been possible to calculate the number of S1 nuclease-sensitive sites induced as a result of exposure to ionizing radiation. These sites were found to occur with a frequency about twice that of the double strand break and are thought to result from a primary ionization event in the DNA.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Base Damage ; Dna ; Nuclease-sensitive Sites ; Yeast
Sprache englisch
Veröffentlichungsjahr 1984
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0955-3002
e-ISSN 1362-3095
Zeitschrift International Journal of Radiation Biology
Quellenangaben Band: 45, Heft: 5, Seiten: 497-504 Artikelnummer: , Supplement: ,
Verlag Informa Healthcare
Begutachtungsstatus Peer reviewed
Institut(e) Abteilung für Strahlenbiologie und Biophysik
DOI 10.1080/09553008414550701
Scopus ID 0021246537
Erfassungsdatum 1984-12-31
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