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Effective avidin coating of erythrocytes by disulfide bond formation : Production of a stable rosetting agent for multiple applications.
J. Immunol. Methods 91, 123-128 (1986)
A method is described for covalently coupling avidin to the membrane of glutaraldehyde-treated erythrocytes. The method is based on the use of the heterobifunctional coupling reagent N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) and appears to be more effective than other protein coupling techniques. The resulting avidin-coated red cells are very stable and can be stored for more than 1 year without aggregation or loss of biotin-binding activity. They can be used in various rosetting assays and were tested for T cell depletion or negative cell selection with five biotinylated monoclonal antibodies.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Avidin-biotin; Cell separation; Coating erythrocyte; Rosetting
ISSN (print) / ISBN
0022-1759
e-ISSN
1872-7905
Zeitschrift
Journal of Immunological Methods
Quellenangaben
Band: 91,
Heft: 1,
Seiten: 123-128
Verlag
Elsevier
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institut für Hämatologie