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Schneckenburger, H. ; Reuter, B.W. ; Schoberth, S.M.*

Time-resolved microfluorescence for measuring coenzyme F420 in Methanobacterium thermoautotrophicum.

FEMS Microbiol. Lett. 22, 205-208 (1984)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The time course of photobleaching and the nanosecond fluorescence decay have been measured from microscopic samples of methanogenic bacteria, to our knowledge the first application of these methods in this field. Decay times of about 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin and other fluorescent compounds the intensity of F420 fluorescence was reduced selectively due to photobleaching. This effect, as well as the different decay time constants could be used to discriminate F420 from other fluorescent components. In addition, active and inactive bacterial cells could be differentiated following the course of photobleaching.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter 7-methylpterin ; Autofluorescence ; Fluorescence Decay ; Methanogenic Bacteria ; Photobleaching
ISSN (print) / ISBN 0378-1097
e-ISSN 1574-6968
Zeitschrift FEMS Microbiology Letters
Quellenangaben Band: 22, Heft: 3, Seiten: 205-208 Artikelnummer: , Supplement: ,
Verlag Wiley
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed