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Schlesier, M.* ; Friedrich, B.

In vivo inactivation of soluble hydrogenase of Alcaligenes eutrophus.

Arch. Microbiol. 129, 150-153 (1981)
Verlagsversion DOI PMC
The soluble, NAD+-reducing hydrogenase in intact cells of Alcaligenes eutrophus was inactivated by oxygen when electron donors such as hydrogen or pyruvate were available. The sole presence of either oxygen or oxidizable substrates did not lead to inactivation of the enzyme. Inactivation occurred similarly under autotrophic growth conditions with hydrogen, oxygen and carbon dioxide. The inactivation followed first order reaction kinetics, and the half-life of the enzyme in cells exposed to a gas atmosphere of hydrogen and oxygen (8:2, v/v) at 30° C was 1.5 h. The process of inactivation did not require ATP-synthesis. There was no experimental evidence that the inactivation is a reversible process catalyzed by a regulatory protein. The possibility is discussed that the inactivation is due to superoxide radical anions (O2 -) produced by the hydrogenase itself.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Alcaligenes Eutrophus ; Autotrophic Growth ; Oxygen-dependent Inactivation ; Soluble Hydrogenase
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Zeitschrift Archives of Microbiology
Quellenangaben Band: 129, Heft: 2, Seiten: 150-153 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Mikrobiologie