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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Kinetics of myeloid progenitor cells in human micro long-term bone marrow cultures.
Exp. Hematol. 16, 145-149 (1988)
Hemopoiesis was analyzed in a miniaturized long-term culture of human bone marrow cells by quantifying the production of granulocyte-macrophage progenitor cells. As in the conventional long-term culture system, hemopoiesis was dependent on the presence of a marrow-derived adherent layer. Adipocytes proved to be essential for long-term proliferation. Optimal growth conditions were maintained by incubation in McCoy's medium supplemented with hydrocortisone, fetal calf serum, and horse serum. When calculated back to the volume of conventional cultures, the numbers and kinetics of nucleated cells and granulocyte-macrophage colony-forming cells were comparable in both culture systems. The microsystem is therefore suitable for performing multiple analyses on small samples of cells.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0301-472X
e-ISSN
0301-472X
Zeitschrift
Experimental Hematology
Quellenangaben
Band: 16,
Heft: 2,
Seiten: 145-149
Verlag
Elsevier
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institut für Hämatologie