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Frankenberg, D. ; Goodhead, D.T.* ; Frankenberg-Schwager, M. ; Harbich, R. ; Bance, D.A. ; Wilkinson, R.E.

Effectiveness of 1·5 kev aluminium k and 0·3 kev carbon k characteristic x-rays at inducing DNA double-Strand breaks in yeast cells.

Int. J. Radiat. Biol. 50, 727-741 (1986)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Induction of DNA double-strand breaks in diploid wild-type yeast cells, and inactivation of diploid mutant cells (rad54-3) unable to repair DNA double-strand breaks, were studied with aluminium K (1·5 keV) and carbon K (0·278 keV) characteristic X-rays. The induction of DNA double-strand breaks was found to increase linearly with absorbed dose for both characteristic X-rays. Carbon K X-rays were more effective than aluminium K X-rays. Relative to 60Co γrays the r.b.e-values for the induction of DNA double-strand breaks were found to be 3·8 and 2·2 for carbon K and aluminium K X-rays respectively. The survival curves of the rad54-3 mutant cells were exponential for both ultrasoft X-rays. For inactivation of rad54-3 mutant cells, the r.b.e.-values relative to 60Co γrays were 2·6 and 2·4 for carbon K and aluminium K X-rays, respectively. The DNA double-strand break data obtained with aluminium K and carbon K X-rays are in agreement with the data obtained for gene mutation, chromosome aberrations and inactivation of mammalian cells, suggesting that DNA double-strand breaks are the possible molecular lesions leading to these effects.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Double-strand Breaks ; Rad54-3 ; Ultrasoft X-rays ; Yeast Cell Inactivation ; Yeast Mutant
ISSN (print) / ISBN 0955-3002
e-ISSN 1362-3095
Zeitschrift International Journal of Radiation Biology
Quellenangaben Band: 50, Heft: 4, Seiten: 727-741 Artikelnummer: , Supplement: ,
Verlag Informa Healthcare
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Abteilung Biophysikalische Strahlenforschung