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Pohlit, W. ; Heyder, I.R.

The shape of dose-survival curves for mammalian cells and repair of potentially lethal damage analyzed by hypertonic treatment.

Radiat. Res. 87, 613-634 (1981)
Verlagsversion DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
During the usual procedure of testing cell survival by colony-forming ability, repair of potentially lethal damage (PLD) takes place. By incubating the cells in hypertonic suspension a certain part of this repair can be inhibited, leading to an exponential dose-survival curve as expected from the Poisson distribution of lethal events in the cells. If such a hypertonic treatment is performed after increasing intervals following irradiation with X rays, curves with increasing shoulder length are obtained. Quantitative analysis of the kinetics of this repair shows that PLD is repaired for about 1 hr after irradiation by a saturated repair system which eliminates about one lesion per 15 min per cell independent of the applied absorbed dose. PLD not eliminated by this 'fast' system is repaired by an unsaturated system with a time constant of several hours. Repair of PLD after X irradiation proceeds quantitatively in this way in plateau-phase cells suspended in a conditioned medium, which seems optimal for such repair. If these cells are suspended after irradiation in normal nutrient medium a certain fraction of the PLD is transformed into irreparable damage. The final survival after repair in nutrient medium is then identical with that obtained by the usual measurement of colony-forming ability on nutrient agar. This indicates that the shoulder in dose-survival curves for plateau-phase cells is partly due to repair of PLD and partly due to manifestation of this damage during repair time.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0033-7587
e-ISSN 1938-5404
Zeitschrift Radiation Research
Quellenangaben Band: 87, Heft: 3, Seiten: 613-634 Artikelnummer: , Supplement: ,
Verlag Radiation Research Society
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Abteilung Biophysikalische Strahlenforschung