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Probst, I. ; Moura, J.J.G.* ; Moura, I.* ; Bruschi, M.H.* ; Le Gall, J.Y.*

Isolation and characterization of a rubredoxin and an (8Fe-8S) ferredoxin from Desulfuromonas acetoxidans.

Biochim. Biophys. Acta 502, 38-44 (1978)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A two cluster (4Fe4S) ferredoxin and a rubredoxin have been isolated from the sulfur-reducing bacterium Desulfuromonas acetoxidans. Their amino acid compositions are reported and compared to those of other iron-sulfur proteins. The ferredoxin contains 8 cysteine residues, 8 atoms of iron and 8 atoms of labile sulfur per molecule; its minimum molecular weight is 6163. The protein exhibits an absorbance ratio of A385 A283 = 0.74. Storage results in a bleaching of the chromophore; the denatured ferredoxin is reconstitutable with iron and sulfide. The instability temperature is 52°C. The rubredoxin does not differ markedly from rubredoxins from other anaerobic bacteria.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0006-3002
Zeitschrift Biochimica et Biophysica Acta
Quellenangaben Band: 502, Heft: 1, Seiten: 38-44 Artikelnummer: , Supplement: ,
Verlag Elsevier
Institut(e) Institut für Mikrobiologie