PuSH - Publikationsserver des Helmholtz Zentrums München

Frings, W. ; Schlegel, H.G.

Synthesis of C4-dicarboxylic acids from pyruvate by Hydrogenomonas eutropha strain H 16

Zur Synthese von C4-Dicarbonsäuren aus Pyruvat durch Hydrogenomonas eutropha Stamm H 16.

Arch. Microbiol. 79, 204-219 (1971)
Verlagsversion DOI PMC
1. Crude extracts and partially purified enzyme preparations obtained from Hydrogenomonas eutropha strain H 16 have been tested for enzymes carboxylating pyruvate and phosphoenolpyruvate. Radiometric methods employing 14C-bicarbonate have been used for the determination of enzyme activities; due to the presence of high activities of NADH oxidase coupled optical tests were unsatisfactory. 2. The rates of carbon dioxide fixation are dependent on the growth substrate of the cells (fructose, lactate, succinate, and hydrogen + carbon dioxide). 3. The activities of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase are high. By acetyl coenzyme A the latter enzyme was stimulated by a factor of four or five. 4. The presence of a biotin dependent pyruvate carboxylase could be excluded, while under the same experimental conditions this enzyme could be determined in yeast. 5. The results are discussed with respect to similar investigations in other autotrophic microorganisms.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Zeitschrift Archives of Microbiology
Quellenangaben Band: 79, Heft: 3, Seiten: 204-219 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Biologie