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Linke, R.P. ; Bommer, J. ; Ritz, E.R. ; Waldherr, R. ; Eulitz, M.

Amyloid kidney stone of uremic patients consist of Beta2-microglobulin fragments.

Biochem. Biophys. Res. Commun. 136, 665-671 (1986)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Urinary stones with amyloid structure, obtained from uremic patients, were analyzed according to molecular weight, amino acid sequence, and antigenic content. A major protein of approximately 7 kD, designated AB protein, was isolated by size exclusion using HPLC in 60% formic acid. AB protein reacted in immunodiffusion only with an antiserum to β2-microglobulin, with β2m spurring over AB protein. N-terminal amino acid sequence analysis defined two fragments homologous to β2m. One fragment commenced with Ile at position 7 and the other with Ser at position 20, with a cleavage point subsequent to a lysyl residue in both. It is concluded that β2m is a precursor of urinary amyloid stones and intratubular concretions of patients with preterminal and terminal renal failure; limited proteolysis is involved in AB amyloid generation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Zeitschrift Biochemical and Biophysical Research Communications
Quellenangaben Band: 136, Heft: 2, Seiten: 665-671 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Immunologie