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Unterberger, C. ; Hanson, S.* ; Klingenhoff, A.* ; Oesterle, D.* ; Frankenberger, M. ; Endo, Y.* ; Matsushita, M.* ; Fujita, T.* ; Schwaeble, W.* ; Weiss, E.H.* ; Ziegler-Heitbrock, L. ; Stover, C.*

Stat3 is involved in control of MASP2 gene expression.

Biochem. Biophys. Res. Commun. 364, 1022-1025 (2007)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Little is known about determinants regulating expression of Mannan-binding lectin associated serine protease-2 (MASP-2), the effector component of the lectin pathway of complement activation. Comparative bioinformatic analysis of the MASP2 promoter regions in human, mouse, and rat, revealed conservation of two putative Stat binding sites, termed StatA and StatB. Site directed mutagenesis specific for these sites was performed. Transcription activity was decreased 5-fold when StatB site was mutated in the wildtype reporter gene construct. Gel retardation and competition assays demonstrated that proteins contained in the nuclear extract prepared from HepG2 specifically bound double-stranded StatB oligonucleotides. Supershift analysis revealed Stat3 to be the major specific binding protein. We conclude that Stat3 binding is important for MASP2 promoter activity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Effector component; Lectin pathway; Promoter
Sprache englisch
Veröffentlichungsjahr 2007
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Zeitschrift Biochemical and Biophysical Research Communications
Quellenangaben Band: 364, Heft: 4, Seiten: 1022-1025 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Lung Health and Immunity (LHI)
POF Topic(s) 30202 - Environmental Health
PSP-Element(e) G-520200-001
PubMed ID 17971300
DOI 10.1616/j.bbrc.2007.10.114
WOS ID 000251358500050
Erfassungsdatum 2007-12-31
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