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Kummer, U. ; Thiel, E.V. ; Doxiadis, I.I.N. ; Eulitz, M. ; Sladoljev, S. ; Thierfelder, S.S.

Tritium radiolabeling of antibodies to high specific activity with N-succinimidyl[2,3-3H]propionate: Use in detecting monoclonal antibodies.

J. Immunol. Methods 42, 367-374 (1981)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A simple method has been established for tritium labeling immunoglobulins. This label avoids certain disadvantages of 125iodine such as high radiation hazard and requirement for frequent labeling owing to the short half-life of this isotope. High specific activities (range 1.8-9.0 cpm/μg protein) were obtained with no loss of functional activity as demonstrated by passive hemagglutination. The labeled conjugate is especially suitable for detection of activity in hybridoma supernatants by indirect binding assay. Adapted to microtiter plates, the method is suitable for large numbers of samples, e.g. up to 800 supernatants may be examined daily. The utility and advantages of the binding assay with respect to reproductibility, sensitivity, binding kinetics, easy and rapid performance are described.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0022-1759
e-ISSN 1872-7905
Zeitschrift Journal of Immunological Methods
Quellenangaben Band: 42, Heft: 3, Seiten: 367-374 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed