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DNA repair genes of Saccharomyces cerevisiae: Complementing rad4 and rev2 mutations by plasmids which cannot be propagated in Escherichia coli.
Curr. Genet. 11, 205-210 (1986)
The RAD4 gene of yeast required for the incision step of DNA excision repair and the REV2 (= RAD5) gene involved in mutagenic DNA repair could not be isolated from genomic libraries propagated in E. coli regardless of copy number of the shuttle vector in yeast. Transformants with plasmids conferring UV resistance to a rad4-4 or a rev2-1 mutant were only recovered if yeast was transformed directly without previous amplification of the gene bank in E. coli. DNA preparations from these yeast clones yielded no transformants in E. coli but retransformation of yeast was possible. This lead to the isolation of a defective derivative of the rad4 complementing plasmid. The modified plasmid was now capable of transforming E. coli but still interfered significantly with its growth.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Yeast; Excision repair; Mutagenic DNA repair; RAD4 and REV2 gene cloning
ISSN (print) / ISBN
0172-8083
e-ISSN
1432-0983
Zeitschrift
Current Genetics
Quellenangaben
Band: 11,
Heft: 3,
Seiten: 205-210
Verlag
Springer
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Abteilung für Strahlenbiologie und Biophysik