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Comparison of the automated KRYPTOR chromogranin A assay with the DAKO ELISA.
Clin. Lab. 60, 2103-2106 (2014)
Background: Currently available assays for the widely used marker for neuroendocrine tumors chromogranin A have a weak comparability, involve manual work, and require batch processing of the samples. In this study, we evaluated the automated chromogranin A KRYPTOR assay compared with the widely used DAKO ELISA. Methods: 83 samples were measured with the DAKO ELISA (EDTA plasma) and the KRYPTOR assay (serum), since different sample materials are recommended. Furthermore, different sample materials were compared. Results: The results between the two assays were highly correlated KRYPTOR serum (ng/mL) = 2.887 ELISA EDTA (U/L) + 5.028; r = 0.99). The inter-assay variation for the KRYPTOR assay was determined as 5.56% at low (95 ng/mL; n = 34) and 6.21% at high (530 ng/mL; n = 33) chromogranin A concentrations. EDTA plasma samples revealed significantly lower results than serum in the KRYPTOR assays and could not be used. Conclusions: In conclusion, the new chromogranin A KRYPTOR assay is fast, reliable, and compares well to an established test after adaptation of sample material and reported units.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
1.084
0.480
4
3
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Chromogranin A ; Assay ; Kryptor ; Tumor Marker; Tumors
Sprache
englisch
Veröffentlichungsjahr
2014
HGF-Berichtsjahr
2015
ISSN (print) / ISBN
1433-6510
Zeitschrift
Clinical Laboratory
Quellenangaben
Band: 60,
Heft: 12,
Seiten: 2103-2106
Verlag
Clinical Laboratory Publ.
Verlagsort
Mainz
Begutachtungsstatus
Peer reviewed
POF Topic(s)
90000 - German Center for Diabetes Research
Forschungsfeld(er)
Helmholtz Diabetes Center
PSP-Element(e)
G-502400-001
PubMed ID
25651748
WOS ID
WOS:000347667300021
Erfassungsdatum
2015-02-16