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Wolf, M.* ; Riedlinger, I.* ; Lehmann, R. ; Häring, H.-U. ; Schleicher, E. ; Peter, A.

Comparison of the automated KRYPTOR chromogranin A assay with the DAKO ELISA.

Clin. Lab. 60, 2103-2106 (2014)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Background: Currently available assays for the widely used marker for neuroendocrine tumors chromogranin A have a weak comparability, involve manual work, and require batch processing of the samples. In this study, we evaluated the automated chromogranin A KRYPTOR assay compared with the widely used DAKO ELISA. Methods: 83 samples were measured with the DAKO ELISA (EDTA plasma) and the KRYPTOR assay (serum), since different sample materials are recommended. Furthermore, different sample materials were compared. Results: The results between the two assays were highly correlated KRYPTOR serum (ng/mL) = 2.887 ELISA EDTA (U/L) + 5.028; r = 0.99). The inter-assay variation for the KRYPTOR assay was determined as 5.56% at low (95 ng/mL; n = 34) and 6.21% at high (530 ng/mL; n = 33) chromogranin A concentrations. EDTA plasma samples revealed significantly lower results than serum in the KRYPTOR assays and could not be used. Conclusions: In conclusion, the new chromogranin A KRYPTOR assay is fast, reliable, and compares well to an established test after adaptation of sample material and reported units.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Chromogranin A ; Assay ; Kryptor ; Tumor Marker; Tumors
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 1433-6510
Zeitschrift Clinical Laboratory
Quellenangaben Band: 60, Heft: 12, Seiten: 2103-2106 Artikelnummer: , Supplement: ,
Verlag Clinical Laboratory Publ.
Verlagsort Mainz
Begutachtungsstatus Peer reviewed
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502400-001
PubMed ID 25651748
Erfassungsdatum 2015-02-16