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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Microarray analysis of small non-coding RNAs.
Methods Mol. Biol. 1296, 161-171 (2015)
Microarray technology has evolved to efficiently profile the expression of RNAs. However, analysis of small non-coding RNAs (ncRNAs) is challenging due to their short length and highly divergent sequences with large variation in GC content leading to very different hybridization properties. To overcome these challenges, LNA-modified oligonucleotides have been used to enhance and normalize the melting temperature (Tm) of capture probes, which allows sensitive profiling of small ncRNAs regardless of their sequence. Here, we describe the isolation and labeling of small non-coding RNAs, as well as their hybridization to microarrays with LNA-modified oligonucleotide probes using a semi-automated hybridization device.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Rederstorff, M.*
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Small Non-Coding RNAs : Methods and Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1296,
Seiten: 161-171
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Cancer (IDC)