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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Qualitative and quantitative proteomic analysis of Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Methods Mol. Biol. 1295, 109-115 (2015)
Formalin-fixed, paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen tissue for retrospective protein biomarker discovery. However, during the formalin fixation proteins undergo degradation and cross-linking, making conventional protein analysis technologies challenging. Cross-linking is even more challenging when quantitative proteome analysis of FFPE tissue is planned. The use of conventional protein labeling technologies on FFPE tissue has turned out to be problematic as the lysine residue labeling targets are frequently blocked by the formalin treatment. We have established a qualitative and quantitative proteomics analysis technique for FFPE tissues that combines label-free proteomic analysis with optimized protein extraction and separation conditions.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Posch, A.*
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Proteomic Profiling : Methods and Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1295,
Seiten: 109-115
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Radiation Biology (ISB)