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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.
RNA Biol. 12, 233-237 (2015)
Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Ash1 Mrna ; Myo4p ; Rna Localization ; She2p ; She3p ; Budding Yeast ; In Vitro Reconstitution ; Mrnp ; Macromolecular Assembly ; Myosin; Class-v Myosin; Messenger-rna; Binding Protein; Saccharomyces-cerevisiae; Translational Repression; Monomeric Myosin; Budding Yeast; Transport; Localization; Myo4p
ISSN (print) / ISBN
1547-6286
e-ISSN
1555-8584
Zeitschrift
RNA Biology
Quellenangaben
Band: 12,
Heft: 3,
Seiten: 233-237
Verlag
Landes Bioscience
Verlagsort
Philadelphia
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Structural Biology (STB)