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Kellner, M. ; Rohrmoser, M. ; Forne, I.* ; Voss, K. ; Burger, K. ; Mühl, B. ; Gruber-Eber, A. ; Kremmer, E. ; Imhof, A.* ; Eick, D.

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex.

Exp. Cell Res. 334, 146-159 (2015)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
PeBoW, a trimeric complex consisting of pescadillo (Pes1), block of proliferation (Bop1), and the WD repeat protein 12 (WDR12), is essential for processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. Applying a mass spectrometric analysis, we identified the DEAD-box helicase DDX27 as stably associated factor of the PeBoW-complex. DDX27 interacts with the PeBoW-complex via an evolutionary conserved FxF motif in the N-terminal domain and is recruited to the nucleolus via its basic C-terminal domain. This recruitment is RNA-dependent and occurs independently of the PeBoW-complex. Interestingly, knockdown of DDX27, but not of Pes1, induces the accumulation of an extended form of the primary 47S rRNA. We conclude that DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3' end formation of 47S rRNA independently of the PeBoW-complex.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Dead-box Helicase ; Fxf Motif ; Nucleolus ; Pebow-complex ; Rrna Processing; Cell-proliferation; Polymerase-i; Terminal Domain; Biogenesis; Protein; Yeast; Transcription; Bop1; Wdr12; Pes1
Sprache englisch
Veröffentlichungsjahr 2015
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 1090-2422
e-ISSN 0014-4827
Zeitschrift Experimental Cell Research
Quellenangaben Band: 334, Heft: 1, Seiten: 146-159 Artikelnummer: , Supplement: ,
Verlag Academic Press
Verlagsort Orlando, Fla.
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Molecular Immunology (IMI)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501400-006
G-501490-001
G-501793-001
PubMed ID 25825154
DOI 10.1016/j.yexcr.2015.03.017
WOS ID WOS:000354911100017
Scopus ID 84929267558
Scopus ID 84926169814
Erfassungsdatum 2015-04-02
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