PuSH - Publikationsserver des Helmholtz Zentrums München: Quantification of regenerative potential in primary human mammary epithelial cells.

Navigation

Startseite

English

Recherche

Erweiterte Suche

Durchblättern nach ...

... Zeitschriften

... Publikationstypen

... Forschungsdaten

... Erscheinungsjahr

Publikationen im Überblick

Hilfe & Kontakt

Ansprechpartner

Hilfe

Datenschutz

Linnemann, J. ; Miura, H. ; Meixner, L.K. ; Irmler, M. ; Kloos, U. ; Hirschi, B. ; Bartsch, H.S.* ; Sass, S. ; Beckers, J. ; Theis, F.J. ; Gabka, C.* ; Sotlar, K.* ; Scheel, C.

Quantification of regenerative potential in primary human mammary epithelial cells.

Development 142, 3239-3251 (2015)

Verlagsversion

DOI

PMC

	Open Access Gold (Paid Option)

Abstract
Metriken
Zusatzinfos

We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49f(hi)/EpCAM(-) population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis.

Altmetric

Weitere Metriken?

[➜Einloggen]