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Kiefer, E. ; Heller, W. ; Ernst, D.

A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites.

Plant Mol. Biol. 18, 33-39 (2000)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides. The method is based on the Nucleon PhytoPure™ system without the use of phenol. The procedure can be completed within 1 h and many samples can be processed at the same time. The yield ranged from 240 μg up to 3 mg per gram of tissue with an average purity measured as A260/280 of 1.85. The RNA was of sufficient quality for use in RT-PCR reactions. Quantitation of single-stranded cDNA was carried out with the RiboGreen™ reagent and of PCR products with the PicoGreen™ reagent.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0167-4412
e-ISSN 0167-4412
Zeitschrift Plant Molecular Biology
Quellenangaben Band: 18, Heft: , Seiten: 33-39 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)