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Kiefer, E. ; Heller, W. ; Ernst, D.

A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites.

Plant Mol. Biol. 18, 33-39 (2000)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides. The method is based on the Nucleon PhytoPure^™ system without the use of phenol. The procedure can be completed within 1 h and many samples can be processed at the same time. The yield ranged from 240 μg up to 3 mg per gram of tissue with an average purity measured as A_260/280 of 1.85. The RNA was of sufficient quality for use in RT-PCR reactions. Quantitation of single-stranded cDNA was carried out with the RiboGreen^™ reagent and of PCR products with the PicoGreen^™ reagent.

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