PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Vincendeau, M. ; Kramer, S. ; Hadian, K. ; Rothenaigner, I. ; Bell, J.* ; Hauck, S.M. ; Bickel, C.* ; Kremmer, E. ; Leib-Mösch, C. ; Brack-Werner, R.

A novel family of host cell factors that control hiv production by targeting rev.

Vortrag: 34th Annual Meeting on Retroviruses in CSHL, 17th May 2009, CSHL, NY, USA. (2009)
HIV-1 Rev is a key inducer of virus replication. Rev facilitates different post-transcriptional events required for the production of viral proteins and RNA genomes. We previously identified a novel Rev-interacting protein termed Risp that is capable of modulating the expression of Revdependent reporter genes. In this report we investigated production of Risp-proteins in lysates of human astrocytes, which are known to restrict HIV production and to suppress Rev activity. We identified several Rispproteins of different sizes. Three of these proteins were shown to interact with Rev in an affinity chromatography–based assay for capture of Revinteractors. Rev shows abnormal cytoplasmic accumulation in astrocytes and we found that Risp proteins also localize to the cytoplasm. RNAi knock-down of Risp expression increased nuclear levels of Rev, suggesting that Risp proteins retain Rev in the cytoplasm. Overexpression and RNAi analyses in a persistently HIV-infected astrocyte model showed negative regulation of HIV replication by Rispproteins. Altogether, we demonstrate that proteins of the Risp-family can interact with Rev and strongly inhibit HIV-1 replication, identifying the first host-cell factors that control HIV production by targeting Rev.
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Sonstiges: Vortrag
Sprache englisch
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 0
Konferenztitel 34th Annual Meeting on Retroviruses in CSHL
Konferzenzdatum 17th May 2009
Konferenzort CSHL, NY, USA
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-502700-001
Erfassungsdatum 2009-12-31
[➜Korrektur melden]