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Ernst, D. ; Kiefer, E.* ; Drouet, A.* ; Sandermann, H.

A simple method of DNA extraction from soil for detection of composite transgenic plants.

Plant Mol. Biol. Rep. 14, 143-148 (1996)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
This new and simple method of DNA extraction from composite soil allows the isolation of plant DNA with high efficiency, quality and reproductivity. The method is based on a simple CaCl2-precipitation step and requires no additional purification steps to eliminate humic acids. The extracted DNA was obtained in sufficient purity and quantity to allow direct detection of transgenes by PCR. Furthermore, the simple procedure allows the assay of many samples at the same time.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0735-9640
e-ISSN 0735-9640
Zeitschrift Plant Molecular Biology Reporter
Quellenangaben Band: 14, Heft: 2, Seiten: 143-148 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)