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Eckey-Kaltenbach, H. ; Großkopf, E.* ; Sandermann, H. ; Ernst, D.

Induction of pathogen defence genes in parsley (Petroselinum crispim L.) plant by ozone.

Proc. R. Soc. Edinburgh, Sect. A 102, 63-74 (1994)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Parsley (Petroselinum crispum L.) is known to respond to pathogen attack by the synthesis of furanocoumarins and to UV-irradiation by the synthesis of flavone glycosides, whereas ozone treatment results in the induction of both pathways. Ozone treatment (200 nl 1−1, 10 h) of parsley seedlings results in an increased mRNA level of early genes within 3 h [pathogenesis related proteins PR1, PR2 and an elicitor-induced protein with unknown function (Eli 16)], of intermediate induced genes within 6 h [phenylalanine ammonia-lyase (PAL), 4-coumaroyl-CoA ligase (4CL), chalcone synthase (CHS)], and of late genes within 12 h [hydroxyproline-rich glycoprotein (HRGP), peroxidase (POD)]. 2D-PAGE of in vitro translated poly(A)+ RNA isolated from ozone-treated parsley seedlings revealed about 20 induced and 10 repressed translation products. A cDNA library from parsley seedlings was differential screened, yielding several induced cDNA clones. One of the ozone-induced cDNA clones could be identified as coding for PR1-1 by hybrid-selected in vitro translation and by DNA sequence analysis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0308-2105
e-ISSN 1473-7124
Zeitschrift Proceedings of the Royal Society of Edinburgh, Section A: Mathematics
Quellenangaben Band: 102, Heft: , Seiten: 63-74 Artikelnummer: , Supplement: ,
Verlag Cambridge Univ. Press
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)