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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Ozone-induced metabolic changes in Scots pine (Pinus sylvestris L.): mRNA isolation and analysis of in vitro translated proteins.
Can. J. For. Res. 24, 2030-2033 (1994)
A rapid isolation procedure for intact poly(A)+ RNA from Scots pine (Pinussylvestris L.) is described. Translation products of poly(A)+ RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 μL/L) for 8 h were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Both qualitative and quantitative differences in the translation products were observed after ozone fumigation. Expression of specific mRNAs, coding for stress-induced polypeptides between 12 kD and 50 kD, was demonstrated after 8 h of ozone treatment.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0045-5067
Zeitschrift
Canadian Journal of Forest Research
Quellenangaben
Band: 24,
Seiten: 2030-2033
Verlag
NRC Research Press
Verlagsort
Ottawa, Ont.
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Biochemical Plant Pathology (BIOP)