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Bi, Z. ; Merl-Pham, J. ; Uehlein, N.* ; Zimmer, I. ; Mühlhans, S. ; Aichler, M. ; Walch, A.K. ; Kaldenhoff, R.* ; Palme, K.* ; Schnitzler, J.-P. ; Block, K.

RNAi-mediated downregulation of poplar Plasma membrane Intrinsic Proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.

J. Proteomics 128, 321-332 (2015)
Postprint DOI PMC
Open Access Green
Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement. Biological significance The present work is a comprehensive survey combining leaf plasma membrane proteomics and physiological methods to assess the functionality of the whole PIP subfamily in tree model system.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Pip ; Populus×canescens ; Aquaporin ; Photosynthesis ; Plasma Membrane Intrinsic Protein ; Plasma Membrane Proteomics ; Stomatal Movement
ISSN (print) / ISBN 1874-3919
e-ISSN 1876-7737
Zeitschrift Journal of Proteomics
Quellenangaben Band: 128, Heft: , Seiten: 321-332 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Environmental Simulation (BIOP-EUS)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)