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Merl-Pham, J. ; Gruhn, F. ; Hauck, S.M.

Proteomic profiling of cigarette smoke induced changes in retinal pigment epithelium cells.

Adv. Exp. Med. Biol. 854, 785-791 (2016)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Age-related macular degeneration (AMD) is a medical condition usually affecting older adults and resulting in a loss of vision in the macula, the center of the visual field. The dry form of this disease presents with atrophy of the retinal pigment epithelium, resulting in the detachment of the retina and loss of photoreceptors. Cigarette smoke is one main risk factor for dry AMD and increases the risk of developing the disease by three times. In order to understand the influence of cigarette smoke on retinal pigment epithelial cells, cultured human ARPE-19 cells were treated with cigarette smoke extract for 24 h. Using quantitative mass spectrometry more than 3000 proteins were identified and their respective abundances were compared between cigarette smoke-treated and untreated cells. Altogether 1932 proteins were quantified with at least two unique peptides, with 686 proteins found to be significantly differentially abundant with p > 0.05. Of these proteins the abundance of 64 proteins was at least 2-fold down-regulated after cigarette smoke treatment while 120 proteins were 2-fold up-regulated. The analysis of associated biological processes revealed an alteration of proteins involved in RNA processing and transport as well as extracellular matrix remodelling in response to cigarette smoke treatment.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Bowes Rickman, C.* ; LaVail, M.M.* ; Anderson, R.E.* ; Grimm, C.* ; Hollyfield, J.G.* ; Ash, J.*
Korrespondenzautor
Schlagwörter Age-related Macular Degeneration ; Cell Fractionation ; Cigarette Smoke ; Quantitative Mass Spectrometry ; Retinal Pigment Epithelium; Macular Degeneration; Label-free; Identification; Exposure; Disease; Stress
ISSN (print) / ISBN 0065-2598
Konferenztitel Retinal Degenerative Diseases : Mechanisms and Experimental Therapy
Zeitschrift Advances in Experimental Medicine and Biology
Quellenangaben Band: 854, Heft: , Seiten: 785-791 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort New York
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)