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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Analyzing illumina gene expression microarray data obtained from human whole blood cell and blood monocyte samples.
Methods Mol. Biol. 1368, 85-97 (2016)
Microarray profiling of gene expression is widely applied to studies in molecular biology and functional genomics. Experimental and technical variations make not only the statistical analysis of single studies but also meta-analyses of different studies very challenging. Here, we describe the analytical steps required to substantially reduce the variations of gene expression data without affecting true effect sizes. A software pipeline has been established using gene expression data from a total of 3358 whole blood cell and blood monocyte samples, all from three German population-based cohorts, measured on the Illumina HumanHT-12 v3 BeadChip array. In summary, adjustment for a few selected technical factors greatly improved reliability of gene expression analyses. Such adjustments are particularly required for meta-analyses of different studies.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Li, P.C.* ; Sedighi, A.* ; Wang, L.*
Schlagwörter
Beadchip ; Gene Expression Analysis ; Human Monocytes ; Human Whole Blood Cells ; Illumina Humanht-12 ; Microarray ; Transcriptomics
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Microarray Technology : Methods and Applications
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1368,
Seiten: 85-97
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Human Genetics (IHG)