PuSH - Publikationsserver des Helmholtz Zentrums München

Ford, S.J. ; Bigliardi, P.L.* ; Sardella, T.C.* ; Urich, A.* ; Burton, N.C.* ; Kacprowicz, M.* ; Bigliardi, M.* ; Olivo, M.* ; Razansky, D.

Structural and functional analysis of intact hair follicles and pilosebaceous units by volumetric multispectral optoacoustic tomography.

J. Invest. Dermatol. 136, 753-761 (2016)
Verlagsversion Postprint Anhang DOI PMC
Open Access Green
Visualizing anatomical and functional features of hair follicle development in their unperturbed environment is key in understanding complex mechanisms of hair pathophysiology and in discovery of novel therapies. Of particular interest is in-vivo visualization of the intact pilosebaceous unit, vascularization of the hair bulb and evaluation of the hair cycle particularly in humans. Furthermore, non-invasive visualization of the sebaceous glands could offer crucial insight into pathophysiology of follicle-related diseases, dry or seborrhoic skin, in particular by combining in-vivo imaging with other phenotyping, genotyping and microbial analyses. The available imaging techniques are limited in their ability for deep tissue in-vivo imaging of hair follicles and lipid-rich sebaceous glands in their entirety without biopsy. We developed a non-invasive, painless and risk-free volumetric multispectral optoacoustic tomography (vMSOT) method for deep tissue three-dimensional visualization of whole hair follicles and surrounding structures with high spatial resolution below 70μm. Herein we demonstrate on-the-fly assessment of key morphometric parameters of follicles and lipid content as well as functional oxygenation parameters of the associated capillary bed. The ease of handheld operation and versatility of the newly-developed approach poise it as an indispensable tool for early diagnosis of disorders of the pilosebaceous unit and surrounding structures, and for monitoring the efficacy of cosmetic and therapeutic interventions.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Free-electron Laser; In-vivo; Gold Nanorods; Selective Photothermolysis; Photoacoustic Tomography; Hemoglobin Concentration; Arthritis; Growth; Skin; Regeneration
ISSN (print) / ISBN 0022-202X
e-ISSN 1523-1747
Zeitschrift Journal of Investigative Dermatology, The
Quellenangaben Band: 136, Heft: 4, Seiten: 753-761 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort New York, NY
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)