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Jung, B. ; Padula, D. ; Burtscher, I. ; Landerer, C. ; Lutter, D. ; Theis, F.J. ; Messias, A.C. ; Geerlof, A. ; Sattler, M. ; Kremmer, E. ; Boldt, K. ; Ueffing, M. ; Lickert, H.

Pitchfork and Gprasp2 target smoothened to the primary cilium for Hedgehog pathway activation.

PLoS ONE 11:e0149477 (2016)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The seven-transmembrane receptor Smoothened (Smo) activates all Hedgehog (Hh) signaling by translocation into the primary cilia (PC), but how this is regulated is not well understood. Here we show that Pitchfork (Pifo) and the G protein-coupled receptor associated sorting protein 2 (Gprasp2) are essential components of an Hh induced ciliary targeting complex able to regulate Smo translocation to the PC. Depletion of Pifo or Gprasp2 leads to failure of Smo translocation to the PC and lack of Hh target gene activation. Together, our results identify a novel protein complex that is regulated by Hh signaling and required for Smo ciliary trafficking and Hh pathway activation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Protein-coupled Receptors; Intraflagellar Transport Proteins; Embryonic Stem-cells; Neuronal Cilia; Localization; Mice; Identification; Ciliopathies; Ciliogenesis; Trafficking
ISSN (print) / ISBN 1932-6203
Zeitschrift PLoS ONE
Quellenangaben Band: 11, Heft: 2, Seiten: , Artikelnummer: e0149477 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort Lawrence, Kan.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Regeneration Research (IDR)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Institute of Structural Biology (STB)
Institute of Molecular Immunology (IMI)
CF Metabolomics & Proteomics (CF-MPC)