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Sattler, C. ; Steer, B. ; Adler, H.

Multiple lytic origins of replication are required for optimal gammaherpesvirus fitness in vitro and in vivo.

PLoS Pathog. 12:e1005510 (2016)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
An unresolved question in herpesvirus biology is why some herpesviruses contain more than one lytic origin of replication (oriLyt). Using murine gammaherpesvirus 68 (MHV-68) as model virus containing two oriLyts, we demonstrate that loss of either of the two oriLyts was well tolerated in some situations but not in others both in vitro and in vivo. This was related to the cell type, the organ or the route of inoculation. Depending on the cell type, different cellular proteins, for example Hexim1 and Rbbp4, were found to be associated with oriLyt DNA. Overexpression or downregulation of these proteins differentially affected the growth of mutants lacking either the left or the right oriLyt. Thus, multiple oriLyts are required to ensure optimal fitness in different cell types and tissues.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Sarcoma-associated Herpesvirus; Dependent Dna-replication; Epstein-barr-virus; Bacterial Artificial Chromosome; Kaposis-sarcoma; Murine Gammaherpesvirus-68; Functional-characterization; Identification; Establishment; Transcription
ISSN (print) / ISBN 1553-7366
e-ISSN 1553-7374
Zeitschrift PLoS Pathogens
Quellenangaben Band: 12, Heft: 3, Seiten: , Artikelnummer: e1005510 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort San Francisco
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)