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Pallua, J.D.* ; Schaefer, G.* ; Bittner, L.K.* ; Pezzei, C.* ; Huck-Pezzei, V.* ; Schoenbichler, S.A.* ; Meding, S. ; Rauser, S. ; Walch, A.K. ; Handler, M.* ; Netzer, M.* ; Osl, M.* ; Seger, M.* ; Pfeifer, B.* ; Baumgartner, C.* ; Lindner, H.* ; Kremser, L.* ; Sarg, B.* ; Klocker, H.* ; Bartsch, G.* ; Bonn, G.K.* ; Huck, C.W.*

Matrix-assisted laser desorption-ionization imaging mass spectrometry for direct tissue analysis.

LC GC N. Am. Suppl. S, Suppl., 21-28 (2011)
Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool in histopathological characterization and represents a modern analytical technique, enabling two-dimensional detection of molecular components of biological samples. Using this method, it is possible to investigate the spatial distribution of proteins, lipids, carbohydrates, cholesterols, nucleic acids, phospholipids, and small molecules in biological systems by in-situ analysis of cell cultures and tissue sections. Recently, MALDI-IMS has become an essential tool for tissue analyses in life science applications, offering global analysis of tissue samples. An advantage of this imaging technique is the acquisition of local molecular expression profiles up to the microscopic level, while maintaining the topographic integrity of the tissue by avoiding time-consuming extraction, purification, or separation steps, respectively. With MALDI-IMS it is possible to determine the distribution of hundreds of unknown compounds in a single measurement, allowing rapid probing of the tissues' biochemistry. Moreover, MALDI-IMS results include qualitative and semiquantitative information, providing unique chemi-morphological information about the tissue status, which represents an important benefit for future analytical interpretation of pathological changes of a tissue. This article summarizes the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter sample preparation; maldi-ms; capture microdissection; small molecules; drug discovery; breast-cancer; ion-trap; proteomics; proteins; desorption/ionization
Sprache englisch
Veröffentlichungsjahr 2011
HGF-Berichtsjahr 2011
ISSN (print) / ISBN 1527-5949
e-ISSN 1939-1889
Zeitschrift LC GC North America
Quellenangaben Band: Suppl. S, Heft: , Seiten: 21-28, Artikelnummer: , Supplement: Suppl.
Verlag Advanstar Communications
Verlagsort Duluth, MN, USA
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-500300-001
G-500390-001
Erfassungsdatum 2011-12-31
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