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Ly, A. ; Buck, A. ; Balluff, B.* ; Sun, N. ; Gorzolka, K. ; Feuchtinger, A. ; Janssen, K.P.* ; Kuppen, P.J.* ; van de Velde, C.J.* ; Weirich, G.* ; Erlmeier, F.* ; Langer, R.* ; Aubele, M. ; Zitzelsberger, H. ; McDonnell, L.A.* ; Aichler, M. ; Walch, A.K.

High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue.

Nat. Protoc. 11, 1428-1443 (2016)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Formalin-fixed and paraffin-embedded (FFPE) tissue specimens are the gold standard for histological examination, and they provide valuable molecular information in tissue-based research. Metabolite assessment from archived tissue samples has not been extensively conducted because of a lack of appropriate protocols and concerns about changes in metabolite content or chemical state due to tissue processing. We present a protocol for the in situ analysis of metabolite content from FFPE samples using a high-mass-resolution matrix-assisted laser desorption/ionization fourier-transform ion cyclotron resonance mass spectrometry imaging (MALDI-FT-ICR-MSI) platform. The method involves FFPE tissue sections that undergo deparaffinization and matrix coating by 9-aminoacridine before MALDI-MSI. Using this platform, we previously detected ∼1,500 m/z species in the mass range m/z 50-1,000 in FFPE samples; the overlap compared with fresh frozen samples is 72% of m/z species, indicating that metabolites are largely conserved in FFPE tissue samples. This protocol can be reproducibly performed on FFPE tissues, including small samples such as tissue microarrays and biopsies. The procedure can be completed in a day, depending on the size of the sample measured and raster size used. Advantages of this approach include easy sample handling, reproducibility, high throughput and the ability to demonstrate molecular spatial distributions in situ. The data acquired with this protocol can be used in research and clinical practice.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Assisted-laser-desorption/ionization; Multivariate Data-analysis; Breast-cancer Tissues; Sample Preparation; Nmr-spectroscopy; Ms Analysis; Matrix; Metabolomics; Specimens; Cells
Sprache
Veröffentlichungsjahr 2016
HGF-Berichtsjahr 2016
ISSN (print) / ISBN 1754-2189
e-ISSN 1750-2799
Zeitschrift Nature Protocols
Quellenangaben Band: 11, Heft: 8, Seiten: 1428-1443 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical Pathology (AAP)
Institute of Pathology (PATH)
Translational Metabolic Oncology (IDC-TMO)
POF Topic(s) 30205 - Bioengineering and Digital Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
Radiation Sciences
PSP-Element(e) G-500390-001
G-500300-001
G-501000-001
DOI 10.1038/nprot.2016.081
WOS ID WOS:000379814300009
Scopus ID 84979912993
PubMed ID 27414759
Erfassungsdatum 2016-07-26
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