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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Studying axonal regeneration by laser microsurgery and high-resolution videomicroscopy.
Methods Mol. Biol. 1451, 271-280 (2016)
Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Fluorescent Protein ; Live Imaging ; Microscopy ; Zebrafish
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1451,
Seiten: 271-280
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed