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Schiener, M. ; Eberlein, B.* ; Moreno-Aguilar, C.* ; Pietsch, G.* ; Serrano, P.* ; McIntyre, M.* ; Schwarze, L.* ; Russkamp, D. ; Biedermann, T.* ; Spillner, E.* ; Darsow, U.* ; Ollert, M.* ; Schmidt-Weber, C.B. ; Blank, S.

Application of recombinant antigen 5 allergens from 7 allergy-relevant hymenoptera species in diagnostics.

Allergy 72, 98-108 (2016)
Verlagsversion Postprint DOI PMC
Open Access Green
BACKGROUND: Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different species can be a diagnostic challenge. To address immunological IgE cross-reactivity on molecular level 7 recombinant antigens 5 of the most important Vespoidea groups were assessed by different diagnostic setups. METHODS: The antigens 5 of yellow jackets, hornets, European and American paper wasps, fire ants, white-faced hornets and Polybia wasps were recombinantly produced in insect cells, immunologically and structurally characterized and their sIgE reactivity assessed by ImmunoCAP, ELISA, cross-inhibition and basophil activation test (BAT) in patients with yellow jacket or Polistes venom allergy of two European geographical areas. RESULTS: All recombinant allergens were correctly folded and structural models and patient reactivity profiles suggested the presence of conserved and unique B cell epitopes. All antigens 5 showed extensive cross-reactivity in sIgE analyses, inhibition assays and BAT. This cross-reactivity was more pronounced in ImmunoCAP measurements with venom extracts than in sIgE analyses with recombinant antigens 5. Dose-response-curves with the allergens in BAT allowed a differentiated individual dissection of relevant sensitization. CONCLUSIONS: Due to extensive cross-reactivity in various diagnostic settings, antigens 5 are inappropriate markers for differential sIgE diagnostics in vespid venom allergy. However, the newly available antigens 5 from further vespid species and the combination of recombinant allergen-based sIgE measurements with BAT represents a practicable way to diagnose clinically relevant sensitization in vespid venom allergy.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Hymenoptera Venom Allergy ; Antigen 5 ; Basophil Activation Test ; Cross-reactivity ; In Vitro Sige Testing; Yellow Jacket Venom; Component-resolved Diagnosis; Fire Ant Venom; Api M 1; Cross-reactivity; Polybia-paulista; Ige Antibodies; Major Allergen; Wasp Venom; Double Positivity
ISSN (print) / ISBN 0105-4538
e-ISSN 1398-9995
Zeitschrift Allergy
Quellenangaben Band: 72, Heft: 1, Seiten: 98-108 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)