Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A nonradioactive assay to measure production and processing of ribosomal RNA by 4sU-tagging.
Methods Mol. Biol. 1455, 121-131 (2016)
In vivo metabolic pulse labeling is a classical approach to assess production and processing of ribosomal RNA (rRNA). However, conventional labeling techniques can be indirect and require work with radioactivity. Here, we describe in detail a protocol for in vivo metabolic labeling, purification, and readout of nascent rRNA by 4-thiouridine (4sU). We propose 4sU labeling as standard nonradioactive technique for the analysis of rRNA metabolism during ribosome biogenesis.
Impact Factor
Scopus SNIP
Scopus
Cited By
Cited By
Altmetric
0.000
0.255
2
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
4-thiouridine ; Click Chemistry ; Metabolic Pulse Labeling ; Nascent Rna ; Ribosome Biogenesis ; Rrna Synthesis
Sprache
englisch
Veröffentlichungsjahr
2016
HGF-Berichtsjahr
2016
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1455,
Seiten: 121-131
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Functional Epigenetics (IFE)
POF Topic(s)
30203 - Molecular Targets and Therapies
Forschungsfeld(er)
Helmholtz Diabetes Center
PSP-Element(e)
G-502890-001
PubMed ID
27576715
Erfassungsdatum
2016-09-02