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Schick, J. ; Seisenberger, C. ; Beig, J. ; Bürger, A. ; Iyer, V.* ; Maier, V. ; Perera, S.* ; Rosen, B.* ; Skarnes, W.C.* ; Wurst, W.

CRISPR-Cas9 enables conditional mutagenesis of challenging loci.

Sci. Rep. 6:32326 (2016)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter One-step Generation; Homologous Recombination; Gene-function; Genome; Cas9; Consortium; Resource; System
Sprache englisch
Veröffentlichungsjahr 2016
HGF-Berichtsjahr 2016
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Zeitschrift Scientific Reports
Quellenangaben Band: 6, Heft: , Seiten: , Artikelnummer: 32326 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)
Institute of Molecular Toxicology and Pharmacology (TOX)
POF Topic(s) 30204 - Cell Programming and Repair
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Genetics and Epidemiology
Enabling and Novel Technologies
PSP-Element(e) G-500500-001
G-505200-001
PubMed ID 27580957
DOI 10.1038/srep32326
WOS ID WOS:000382367900001
Scopus ID 84984868418
Erfassungsdatum 2016-09-05
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