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Meckenstock, R.U. ; Warthmann, R.J.* ; Schäfer, W.*

Inhibition of anaerobic microbial o-xylene degradation by toluene in sulfidogenic sediment columns and pure cultures.

FEMS Microbiol. Ecol. 47, 381-386 (2004)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
It is frequently observed in aromatic hydrocarbons such as benzene, toluene, ethylbenzene, xylene (BTEX)-contaminated aquifers that toluene degrades faster than xylenes and benzene. In sediment column experiments which were run with a mixture of BTEX compounds toluene degradation started after a lag period of several weeks. When we omitted toluene from the culture medium o-xylene degradation started. Xylene degradation could be inhibited by adding toluene back to the medium and could be recovered when toluene was omitted again. This was observed repeatedly when toluene concentrations higher than 20 muM were added. Two sulphate-reducing bacterial species, isolated from the column material, were used to investigate the degradation behaviour in detail. Strain TRMI degraded exclusively toluene, strain OX39 degraded preferentially o-xylene and toluene only after an adaptation period of more than 90 days when added as the sole substrate. Growth and o-xylene degradation of strain OX39 were inhibited by toluene concentrations as low as 40 muM, whereas, in contrast, toluene degradation by strain TRMI was not inhibited by o-xylene concentrations up to 0.5 mM. Both the column data and the batch experiments indicated that two Organisms were responsible for the toluene/xylene degradation in the sediment column. One strain degraded only toluene and was not effected by xylene and the second degraded xylene and was inhibited by toluene. Our findings offer an explanation that the observed differential degradation of BTEX compounds in contaminated aquifers could originate from a partial metabolic inhibition of xylene-degrading organisms by toluene.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter biodegradation; natural attenuation; aromatic hydrocarbon; mixture of aromatic hydrocarbons such as benzene; toluene; ethylbenzene; xylene; polycyclic aromatic hydrocarbon; metabolic inhibition; sulfate-reducing bacterium
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Zeitschrift FEMS Microbiology Ecology
Quellenangaben Band: 47, Heft: 3, Seiten: 381-386 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)